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Sep.2024 06
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Explanation of Aerosol Detection Technology Bioaerosol monitor Analysis of Detection and Equipment Principles
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Aerosols refer to relatively stable dispersed systems formed by solid or liquid particles suspended in the atmosphere. Aerosols can be divided into biological aerosols and non biological aerosols. Bioaerosols include viruses, bacteria and related aggregates, fungi, pollen, and animal and plant debris in the air. The diffusion of biological aerosol particles can affect agriculture, animal and human health, and even affect water circulation and climate.
Analysis of Detection and Equipment Principles----Collect fluorescence generated by organic matter in organisms
1. Detection principle When the fluorescent groups contained in biological particles are irradiated, they will produce specific fluorescence spectra. The main fluorescent groups, such as tryptophan, riboflavin, nicotinamide adenine dinucleotide (NADH), as well as some other metabolites and biomolecules, will also emit fluorescence. By exciting with a UV light source, the fluorescence signal is analyzed to distinguish between fluorescent and non fluorescent particles, thereby obtaining the number of fluorescent particles. 2. Equipment principle Using a UV light source, the beam is collimated and focused through a lens group. The intersection of the centerline of the gas path direction, the optical axis of the excitation light path, and the optical axis of the fluorescence receiving light path is the photosensitive area. The gas flow brings aerosol particles to the photosensitive area, and when the laser is irradiated on the particles, elastic scattering light and fluorescence are generated. A parabolic reflector collects scattered light and fluorescence, and then separates them through a dichroic mirror. Next, scattered light and fluorescence are respectively received by two photomultiplier tubes (PMT). The photomultiplier tube converts the detected light signal into a current signal, which is amplified and analog-to-digital converted before being transmitted to the field programmable gate array (FPGA) for calculation, ultimately obtaining the number of fluorescent particles.
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